The game has a feature that was unusual in side-scrolling platform games at the time, largely to the NES itself. Unlike Sunsoft's Batman for the Mega Drive/Genesis, which features the grappling hook, Batman has the ability to do a wall jump, which is reminiscent of Ryu Hayabusa's wall scaling in Ninja Gaiden. He is also able to use three projectile weapons: the batarang, batdisk, and bat speargun, which are powered by pellet cartridges.
The stages in the game consists of the Gotham City Streets, Axis Chemical Plant, Gotham Sewers, Mysterious Laboratory, and the Gotham Cathedral Belltower. A number of notable DC Comics villains appear as low-level minions in the game, including Deadshot, KGBeast, Maxie Zeus, Heat Wave, Shakedown, and Nightslayer. The bosses in the game are Killer Moth, a device known as the Machine Intelligence System, the Electrocutioner, a machine known as the Dual-Container Alarm, Firebug, and the Joker. The penultimate boss will be skipped in the continued play if the player reached the Joker previously, so the player can directly fight the Joker next time.