As with the previous CSI games, there are five cases to work on. However, the game includes improvements on CSI: 3 Dimensions of Murder, like a 3D crime scene kit. The voice of Sara Sidle is again performed by a soundalike (Kate Savage) and not Jorja Fox. Catherine Willows is also replaced by a soundalike in this game, with Edie Mirman standing in for Marg Helgenberger.