The game begins with a couple of prisoners attempting to escape from the dungeon they are imprisoned in. After breaking out of their cell, they find a locked door. One of the prisoners pulls a nearby lever, which both unlocks the door and releases creatures that were locked in the dungeon. They made it to the hall and approached a door. However, a monstrous masked figure suddenly appears, causing the prisoners to panic and run toward the door. They find it to be locked before the wall closes in and kills them both.
While on vacation in Romania, Josh and his wife Kate are on a train with several other tourists. During the train ride, Kate is about to tell Josh something when she discovers he was hiding a bottle of alcohol. Angry, she walks out and heads to the dining car. Josh comes across a Romanian man who tells him that his wife left a letter for him, and reads it. It says that Kate is sorry for getting angry at Josh, that she wasn't herself lately and asks Josh to meet her at the dining cart. Feeling guilty, Josh heads to the dining car to apologize. On his way, he encounters many travelers, such as Gregor, an elderly man; Lin, a student; Sacha and Tasha, two ballerinas; four teenagers - Monica, Max, Aaron and Katja - heading to a rave; Jane, an English psychiatrist; two Romanian generals; a Romanian ticket collector; a German businessman; and Yeli, a fortune-teller who gives Josh a grim prediction. He then proceeds further, only to find one of the cars is covered in blood. Bursting forth, he finds his wife being carried by a large man, with an odd-looking mechanism on his face. One of the generals tries to stop the man, but is ripped in half. Josh and Yeli follow, catching a glimpse of a strange man laughing maniacally. Suddenly, the train derails, and Josh is knocked unconscious.
Josh awakens to find the train has slid into a river. He tries to save the ticket collector from drowning, but he fails. The general calls him to shore, and Josh runs to a cave where the other survivors are hiding. The businessman walks away from the cave into the forest, where he is killed by something off-screen. Panicking, the survivors run deeper into the cave to escape, until they fall out the other side into a swamp. The other general is then pulled under and killed. After escaping the swamp and walking through the forest, they come upon a cemetery where they meet up with another group of survivors; Aaron, Sacha and Tasha. Lin wanders off into a nearby cellar, and is suddenly heard screaming. Josh runs to help and finds her panicking, saying she saw a corpse move. Josh investigates and the corpse, along with several others, reanimate and attack them both. The girl runs away, and Josh fends them off before pursuing her. Josh exits the cellar and finds that all the survivors have fled due to the zombies. As Josh makes his way through the cemetery, he witnesses Monica being decapitated by Ernst and thrown at a wall. Ernst then subdues Josh, who loses consciousness again.