Super Monkey Ball: Touch & Roll has two different modes: "Party" and "Main". There are six minigames: Race, War, Bowling, Air Hockey, Golf, and Fight. Main has a Challenge Mode in which the player can try to beat the game, or Practice Mode. There are 12 worlds in total, ten of which are available initially and the last two being unlockable.
As in previous Super Monkey Ball games, the object is to roll a monkey from the start of a level to the finish line within an allotted time limit. Along the way, the monkey collects bananas and banana bunches, worth five bananas, for the chance to gain an extra life, after getting ten bananas. Characters include AiAi, MeeMee, Baby and GonGon.